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Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice

Item Type:Article
Title:Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice
Creators Name:Pecher, G. and Harnack, U. and Guenther, M. and Hummel, M. and Fichtner, I. and Schenk, J.A.
Abstract:Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the {alpha}/{beta}-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy.
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:283
Number:4
Page Range:738-742
Date:1 January 2001
Official Publication:https://doi.org/10.1006/bbrc.2001.4846
PubMed:View item in PubMed

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