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Sialyl Lewisx-liposomes as vehicles for site-directed, E-selectin-mediated drug transfer into activated endothelial cells

Item Type:Article
Title:Sialyl Lewisx-liposomes as vehicles for site-directed, E-selectin-mediated drug transfer into activated endothelial cells
Creators Name:Stahn, R. and Grittner, C. and Zeisig, R. and Karsten, U. and Felix, S.B. and Wenzel, K.
Abstract:E-selectin, exclusively expressed on activated endothelial cells, is a potential target for site-directed delivery of agents. We and others have shown that sialyl Lewisx-liposomes (sLex-liposomes) are recognized by E-selectin. We now report an approach employing sLex-liposomes to deliver antisense oligonucleotides (AS-ODNs) directed against the adhesion molecule ICAM-1 to activated vascular endothelial cells. ICAM-I expression was analyzed at the protein level by immunofluorescence and a cell surface ELISA, and at the RNA level by RT-PCR. We have investigated two different AS-ODNs complementary to the 3′ untranslated region and the AUG translation initiation codon of ICAM-1 mRNA. Both inhibited protein expression, but did not influence the mRNA level, pointing to a hybridization of AS-ODNs with the mRNA in the cytoplasm. Our results demonstrate the feasibility of a novel approach for the delivery of agents to activated endothelial cells by glycoliposomes targeted to E-selectin.
Keywords:Antisense Strategy, E-Selectin, ICAM-1, Site-Directed Delivery, sLex-Liposomes
Source:Cellular and Molecular Life Sciences
ISSN:1420-682X
Publisher:Birkhauser Verlag AG (Switzerland)
Volume:58
Number:1
Page Range:141-147
Date:1 January 2001
Official Publication:https://doi.org/10.1007/PL00000774
PubMed:View item in PubMed

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