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Inducible nitric oxide synthase in the myocard

Item Type:Article
Title:Inducible nitric oxide synthase in the myocard
Creators Name:Buchwalow, I.B. and Schulze, W. and Karczewski, P. and Kostic, M.M. and Wallukat, G. and Morwinski, R. and Krause, E.G. and Mueller, J. and Paul, M. and Slezak, J. and Luft, F.C. and Haller, H.
Abstract:Recognition of significance of nitric oxide synthases (NOS) in cardiovascular regulations has led to intensive research and development of therapies focused on NOS as potential therapeutic targets. However, the NOS isoform profile of cardiac tissue and subcellular localization of NOS isoforms remain a matter of debate. The aim of this study was to investigate the localization of an inducible NOS isoform (NOS2) in cardiomyocytes. Employing a novel immunocytochemical technique of a catalyzed reporter deposition system with tyramide and electron microscopical immunocytochemistry complemented with Western blotting and RT-PCR, we detected NOS2 both in rat neonatal and adult cultured cardiomyocytes and in the normal myocard of adult rats as well as in the human myocard of patients with dilative cardiomyopathy. NOS2 was targeted predominantly to a particulate component of the cardiomyocyte - along contractile fibers, in the plasma membrane including T-tubules, as well as in the nuclear envelope, mitochondria and Golgi complex. Our results point to an involvement of NOS2 in maintaining cardiac homeostasis and contradict to the notion that NOS2 is expressed in cardiac tissue only in response to various physiological and pathogenic factors. NOS2 targeting to mitochondria and contractile fibers suggests a relationship of NO with contractile function and energy production in the cardiac muscle.
Keywords:Cardiomyocytes, Immunocytochemistry, Inducible Nitric Oxide Synthase, Animals, Rats
Source:Molecular and Cellular Biochemistry
ISSN:0300-8177
Publisher:Springer (Germany)
Volume:217
Number:1-2
Page Range:73-82
Date:1 January 2001
Official Publication:https://doi.org/10.1023/A:1007286602865
PubMed:View item in PubMed

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