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Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily

Item Type:Article
Title:Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily
Creators Name:Kutay, U. and Hartmann, E. and Treichel, N. and Calado, A. and Carmo-Fonseca, M. and Prehn, S. and Kraft, R. and Goerlich, D. and Bischoff, F.R.
Abstract:Nucleo-cytoplasmic transport comprises a large number of distinct pathways, many of which are defined by members of the importin {beta} superfamily of nuclear transport receptors. These transport receptors all directly interact with RanGTP to modulate the compartment specific binding of their transport substrates. To identify new members of the importin {beta} family, we used affinity chromatography on immobilized RanGTP and isolated Ran-binding protein (RanBP) 16 from HeLa cell extracts. RanBP16 and its close human homologue, RanBP17, are distant members of the importin {beta} family. Like the other members of the transport receptor superfamily, RanBP16 interacts with the nuclear pore complex and is able to enter the nucleus independent of energy and additional nuclear transport receptors.
Keywords:Amino Acid Sequence, Base Sequence, DNA Primers, Hela Cells, Karyopherins, Molecular Sequence Data, Nuclear Proteins, Ran GTP-Binding Protein, Amino Acid Sequence Homology, Animals
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:40163-40168
Date:1 January 2000
Official Publication:https://doi.org/10.1074/jbc.M006242200
PubMed:View item in PubMed

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