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Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes

Item Type:Article
Title:Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes
Creators Name:Madruga, J. and Briegel, K. and Diebold, S. and Boehmelt, G. and Vogel, F. and Zenke, M.
Abstract:The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8α and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.
Keywords:Biological Markers, CD3 Antigens, CD8 Antigens, Cytoplasm, Dendritic Cells, DNA-Binding Proteins, Estrogen Receptors, GATA3 Transcription Factor, Gene Expression, Hematopoietic Stem Cells, Histocompatibility Antigens Class II, Neoplastic Cell Transformation, Oncogene Proteins v-Rel, Recombinant Fusion Proteins, Trans-Activators, Transformed Cell Line, Animals, Chickens
Publisher:Urban & Fischer
Page Range:394-407
Date:1 November 2000
PubMed:View item in PubMed

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