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Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter

Item Type:Article
Title:Cloning and initial analysis of the human multidrug resistance-related MVP/LRP gene promoter
Creators Name:Lange, C. and Walther, W. and Schwabe, H. and Stein, U.
Abstract:The lung resistance-related protein (LRP) was identified as the human major vault protein (MVP), and is overexpressed in various multidrug-resistant cancer cell lines and clinical samples. We characterized DNA sequences upstream to the transcription initiation site of the MVP gene in the human non-small cell lung cancer cell line SW-1573. A 1.9-kb and a shortened 0.7-kb fragment of the 5'-upstream genomic region show strong promoter activity in chloramphenicol acetyltransferase (CAT) reporter assays. The promoter is TATA-less and contains an inverted CCAAT-box and a Sp1 site located near to a p53 binding motif. An alternative 3'-splice site of intron 1 results in a splicing variant within the 5'-untranslated region of MVP mRNA.
Keywords:Gene Promoter, Human Major Vault Protein/Lung Resistance-Related Protein, Multidrug Resistance
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:278
Number:1
Page Range:125-133
Date:11 November 2000
PubMed:View item in PubMed

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