An anchored YAC-STS framework for the rat genome

Item Type:	Article
Title:	An anchored YAC-STS framework for the rat genome
Creators Name:	Cai, L. and Lindpaintner, K. and Browne, J. and Gruetzner, F. and Haaf, T. and James, M.R. and Bihoreau, M.T.
Abstract:	We report here the first YAC-STS framework for the rat genome. A total of 417 anchor microsatellite markers were used to screen a 10-fold redundant YAC library. One or more unambiguous YACs were identified for 372 markers. Assuming the genetic length of the rat genome to be 2,000 cM (Bihoreau et al. 1997b), the YAC-STS framework will provide, on average, one informative YAC clone every 5.4 cM. A total of 111 anchor markers used in this study were derived from known gene regions. We also demonstrated one of the important and immediate uses of this YAC-STS framework, which is to establish a correlation between the genetic and cytogenetic maps in the rat through FISH analysis.
Keywords:	Chromosome Mapping, Chromosomes, Fluorescence In Situ Hybridization, Genome, Microsatellite Repeats, Non-U.S. Gov't Support, Sequence Tagged Sites, U.S. Gov't, P.H.S. Support, Animals, Rats
Source:	Cytogenetics and Cell Genetics
ISSN:	0301-0171
Publisher:	Karger
Volume:	89
Number:	3-4
Page Range:	168-170
Date:	1 January 2000
Official Publication:	https://doi.org/10.1159/000015606
PubMed:	View item in PubMed

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