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Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein

Item Type:Article
Title:Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein
Creators Name:Gedvilaite, A. and Froemmel, C. and Sasnauskas, K. and Micheel, B. and Oezel, M. and Behrsing, O. and Staniulis, J. and Jandrig, B. and Scherneck, S. and Ulrich, R.
Abstract:We generated highly immunogenic virus-like particles that are based on the capsid protein VP1 of the hamster polyomavirus (HaPV-VP1) and harbor inserted foreign epitopes. The HaPV-VP1 regions spanning amino acids 81-88 (position 1), 222/223 (2), 244-246 (3), and 289-294 (4) were predicted to be surface exposed. An epitope of the pre-S1 region of the hepatitis B virus (designated S1; amino acid sequence DPAFR) was introduced into the predicted positions of VP1. All VP1/S1 fusion proteins were expressed in yeast and generated virus-like particles. Immunoassays using the S1-specific monoclonal antibody MA18/7 and immunization of C57B16 mice with different VP1/S1 constructs showed a pronounced reactivity and a strong S1-specific antibody response for particles carrying the insert in position 1, 2, 1+2, and 1+3. Our results suggest that HaPV-VP1 represents a highly flexible carrier moiety for the insertion of foreign sequences offering a broad range of potential uses, especially in vaccine development.
Keywords:Amino Acid Sequence, Capsid, Capsid Proteins, Cricetinae, Enzyme Multiplied Immunoassay Technique, Epitopes, Genetic Vectors, Hepatitis B Surface Antigens, Immunoelectron Microscopy, Inbred C57BL Mice, Insertional Mutagenesis, Molecular Models, Molecular Sequence Data, Monoclonal Antibodies, Peptide Fragments, Polyomavirus, Protein Conformation, Protein Precursors, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Sequence Alignment, Synthetic Vaccines, Viral Antibodies, Viral Antigens, Animals, Mice
Source:Virology
ISSN:0042-6822
Publisher:Academic Press (U.S.A.)
Volume:273
Number:1
Page Range:21-35
Date:20 July 2000
Official Publication:https://doi.org/10.1006/viro.2000.0392
PubMed:View item in PubMed

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