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Profilin I attached to the Golgi is required for the formation of constitutive transport vesicles at the trans-Golgi network

Item Type:Article
Title:Profilin I attached to the Golgi is required for the formation of constitutive transport vesicles at the trans-Golgi network
Creators Name:Dong, J. and Radau, B. and Otto, A. and Mueller, E.C. and Lindschau, C. and Westermann, P.
Abstract:Profilin I was identified, by mass spectrometric sequencing and immunoblotting, as a component of purified Golgi cisternae from HepG2 cells. Binding to the Golgi was verified by indirect immunofluorescence in MT-1 cells showing that a fraction of profilin I colocalizes with TGN38, a marker of the trans-Golgi network (TGN). Studying the formation of constitutive exocytic vesicles at the TGN in a cell-free system demonstrated that cytosolic profilin I has no effect, while incubation of Golgi cisternae with a profilin I-specific antibody reduced vesicle formation by about 50%. Notably, the antibody displaces a fraction of the Golgi-bound dynamin II indicating that profilin I may indirectly promote vesicle formation by supporting the binding of dynamin II to the Golgi membrane. The impact of dynamin II on vesicle formation is demonstrated by incubating the Golgi with the proline-rich domain of dynamin II which concomitantly displaces dynamin II and inhibits vesicle formation. The data provide evidence that profilin I attaches to the Golgi apparatus and is required for the formation of constitutive transport vesicles.
Keywords:Dynamin II, Exocytic Vesicle, Golgi, Human Hepatocellular Carcinoma Cell Line (HepG2), Profilin I, Trans-Golgi Network
Source:Biochimica et Biophysica Acta - Molecular Cell Research
ISSN:0167-4889
Publisher:Elsevier Science BV (The Netherlands)
Volume:1497
Number:2
Page Range:253-260
Date:21 July 2000
Official Publication:https://doi.org/10.1016/S0167-4889(00)00056-2
PubMed:View item in PubMed

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