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A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline

Item Type:Article
Title:A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline
Creators Name:Beliakova, M.M. and Anokhina, M.M. and Spiridonova, V.A. and Dobrov, E.N. and Egorov, T.A. and Wittmann-Liebold, B. and Orth, P. and Saenger, W. and Kopylov, A.M.
Abstract:Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex and allowed characterization of the modified segments of the protein. The potential of tetracycline as a fine modifying reagent was established. In the complex of TetR(D) protein with tetracycline, the antibiotic modifies at least two segments, Ile59-Glu73 and Ala173-Glu183, which form a binding tunnel for the drug according to the X-ray analysis. These data open possibilities for the use of different tetracycline targets for structural studies in solution.
Keywords:Binding Site, Photo-activated Affinity Modification, Protein Sequencing, Tetracycline, Transcription Repressor Protein TetR(D)
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:477
Number:3
Page Range:263-267
Date:21 July 2000
Official Publication:https://doi.org/10.1016/S0014-5793(00)01728-2
PubMed:View item in PubMed

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