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Regionally distinct regulation of astroglial neurotransmitter receptors by fibroblast growth factor-2

Item Type:Article
Title:Regionally distinct regulation of astroglial neurotransmitter receptors by fibroblast growth factor-2
Creators Name:Reuss, B. and Leung, D.S.Y. and Ohlemeyer, C. and Kettenmann, H. and Unsicker, K.
Abstract:Fibroblast growth factor (FGF)-2 is an abundant astroglial cytokine. We have previously shown that FGF-2 down-regulates gap junctions in primary astroglial cultures (B. Reuss et al., 1998, Glia 22, 19-30). We demonstrate now that FGF-2 induces astroglial dopamine (DA) sensitivity and D1 dopamine-receptor (D1DR) antigen and message in cortical and striatal astroglial cultures. On the functional level 10 μmol/L DA triggered transient increases in astroglial [Ca2+]1. In gap-junction-coupled cells, no FGF-2-dependent changes in proportions of DA-responsive cells were observable. However, uncoupling with octanol or 18α-glycirrhetinic acid isolated the smaller population of astrocytes intrinsically sensitive to DA which was significantly increased by FGF-2 in cortical and striatal cultures. Administration of DR-specific substances revealed that FGF-2 upregulated D1DR. These results indicate that downregulation of astroglial gap junctions by FGF-2 is accompanied by an upregulation of D1DR and DA sensitivity, adding a new aspect to the role of FGF-2 in the regulation of brain functions.
Keywords:Astrocytes, Calcium, Cell Count, Cultured Cells, Cerebral Cortex, Corpus Striatum, Dopamine, Dopamine Agonists, Dopamine D1 Receptors, Fibroblast Growth Factor 2, Gap Junctions, Glutamic Acid, Glycyrrhetinic Acid, Intracellular Fluid, Mesencephalon, Messenger RNA, Neurotransmitter Receptors, Newborn Animals, Octanols, Animals, Rats
Source:Molecular and Cellular Neuroscience
Publisher:Academic Press
Page Range:42-58
Date:1 July 2000
Official Publication:https://doi.org/10.1006/mcne.2000.0857
PubMed:View item in PubMed

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