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Allogeneic vaccination for renal cell carcinoma: development and monitoring

Item Type:Article
Title:Allogeneic vaccination for renal cell carcinoma: development and monitoring
Creators Name:Pohla, H. and Frankenberger, B. and Stadlbauer, B. and Oberneder, R. and Hofstetter, A. and Willimsky, G. and Pezzutto, A. and Doerken, B. and Blankenstein, T. and Schendel, D.J.
Abstract:An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shared by many tumors to which patients are not tolerant. A variety of tumor peptides should be presented by different HLA-molecules due to limited MHC matching with recipients and last but not least, the vaccine should have a strong growth potential in vitro to allow adequate amounts of vaccine to be generated for long-term usage. In vitro and in situ studies with the renal cell carcinoma cell line RCC-26 demonstrate: (1) RCC-26 can induce complex allospecific responses through direct priming; (2) RCC-26 can not only reactivate cytotoxic T lymphocytes (CTL) of a memory phenotype but they also can induce de novo tumor-antigen associated responses in normal donors; (3) these cells present epitopes restricted by several MHC molecules, allowing the vaccination of patients matched for different HLA alleles; and (4) they stimulate HLA-A*0201-restricted T cells bearing characteristic T cell receptors (TCR). Thus, in addition to using limiting dilution killer and ELISPOT assays, molecular tracking of a tumor-specific TCR can be used to judge the development of antitumor reactivity and vaccine efficiency.
Keywords:Allogeneic Vaccination, Chromium Release, ELISPOT, Immune Monitoring, Lanthanide Release, Renal Cell Carcinoma, TCR Tracking
Source:Bone Marrow Transplantation
Publisher:Nature Publishing Group
Volume:25 Suppl. 2
Page Range:S83-S87
Date:1 May 2000
Official Publication:http://www.nature.com/bmt/journal/v25/n2s/abs/1702362a.html
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