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Rat gene mapping using PCR-analyzed microsatellites

Item Type:Article
Title:Rat gene mapping using PCR-analyzed microsatellites
Creators Name:Serikawa, T. and Kuramoto, T. and Hilbert, P. and Mori, M. and Yamada, J. and Dubay, C.J. and Lindpaintner, K. and Ganten, D. and Guenet, J.L. and Lathrop, G.M. and Beckmann, J.S.
Abstract:One hundred and seventy-four rat loci which contain short tandem repeat sequences were extracted from the GenBank or EMBL data bases and used to define primers for amplification by the polymerase chain reaction (PCR) of the microsatellite regions, creating PCR-formatted sequence-tagged microsatellite sites (STMSs). One hundred and thirty-four STMSs for 118 loci, including 6 randomly cloned STMSs, were characterized: (i) PCR-analyzed loci were assigned to specific chromosomes using a panel of rat x mouse somatic cell hybrid clones. (ii) Length variation of the STMSs among 8 inbred rat strains could be visualized at 85 of 107 loci examined (79.4%). (iii) A genetic map, integrating biochemical, coat color, mutant and restriction fragment length polymorphism loci, was constructed based on the segregation of 125 polymorphic markers in seven rat backcrosses and in two F2 crosses. Twenty four linkage groups were identified, all of which were assigned to a defined chromosome. As a reflection of the bias for coding sequences in the public data bases, the STMSs described herein are often associated with genes. Hence, the genetic map we report coincides with a gene map. The corresponding map locations of the homologous mouse and human genes are also listed for comparative mapping purposes.
Keywords:Base Sequence, Chromosome Mapping, Satellite DNA, Single-Stranded DNA, Factual Databases, Hybrid Cells, Linkage (Genetics), Molecular Sequence Data, Polymerase Chain Reaction, Genetic Polymorphism, Nucleic Acid Repetitive Sequences, Animals, Mice, Rats
Publisher:Genetics Society Of America
Page Range:701-721
Date:1 July 1992
Official Publication:http://www.genetics.org/cgi/content/abstract/131/3/701
PubMed:View item in PubMed

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