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Nucleolin and YB-1 are required for JNK-mediated interleukin-2 mRNA stabilization during T-cell activation

Official URL:https://doi.org/10.1101/gad.14.10.1236
PubMed:View item in PubMed
Creators Name:Chen, C.Y. and Gherzi, R. and Andersen, J.S. and Gaietta, G. and Juerchott, K. and Royer, H.D. and Mann, M. and Karin, M.
Journal Title:Genes & Development
Journal Abbreviation:Genes Dev
Page Range:1236-1248
Date:15 May 2000
Keywords:JNK, Nucleolin, Stabilization, T-cell Activation, Trans-Acting Factors
Abstract:Regulated mRNA turnover is a highly important process, but its mechanism is poorly understood. Using interleukin-2 (IL-2) mRNA as a model, we described a role for the JNK-signaling pathway in stabilization of IL-2 mRNA during T-cell activation, acting via a JNK response element (JRE) in the 5' untranslated region (UTR). We have now identified two major RNA-binding proteins, nucleolin and YB-1, that specifically bind to the JRE. Binding of both proteins is required for IL-2 mRNA stabilization induced by T-cell activation signals and for JNK-induced stabilization in a cell-free system that duplicates essential features of regulated mRNA decay. Nucleolin and YB. 1 are required for formation of an IL-2 mRNP complex that responds to specific mRNA stabilizing signals.
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Item Type:Article

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