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Normal blood pressure and plasma renin activity in mice lacking the renin-binding protein, a cellular renin inhibitor

Item Type:Article
Title:Normal blood pressure and plasma renin activity in mice lacking the renin-binding protein, a cellular renin inhibitor
Creators Name:Schmitz, C. and Gotthardt, M. and Hinderlich, S. and Leheste, J.R. and Gross, V. and Vorum, H. and Christensen, E.I. and Luft, F.C. and Takahashi, S. and Willnow, T.E.
Abstract:In renal extracts, some renin is present as 'high molecular weight renin,' a heterodimeric complex of renin with the 46-kDa renin-binding protein (RnBP), also known as N-acyl-D-glucosamine 2-epimerase. Because RnBP specifically inhibits renin activity, the protein was proposed to play an important role in the regulation of the renin-angiotensin system (RAS). Using gene targeting, we have generated mice lacking RnBP and tested this hypothesis in vivo. In particular, we analyzed biosynthesis, secretion, and activity of renin and other components of the RAS in mice lacking RnBP. Despite extensive investigations, we were unable to detect any major effects of RnBP deficiency on the plasma and renal RAS or on blood pressure regulation. Contrary to previous hypotheses, we conclude that RnBP does not play a significant role in the regulation of renin activity in plasma or kidney. However, RnBP knockout mice excrete an abnormal pattern of carbohydrates in the urine, indicating a role of the protein in renal carbohydrate metabolism.
Keywords:Blood Pressure, Carbohydrate Epimerases, Carrier Proteins, Cilazapril, Enzyme Precursors, Gene Expression, Kidney, Knockout Mice, Organ Specificity, Reference Values, Renin, Animals, Mice
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:275
Number:20
Page Range:15357-15362
Date:19 May 2000
PubMed:View item in PubMed

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