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Immunocytochemical visualization of transfected DNA in cultured cells

Item Type:Article
Title:Immunocytochemical visualization of transfected DNA in cultured cells
Creators Name:Zaitsev, S. and Buchwalow, I. and Haberland, A. and Tkachuk, S. and Boettger, M.
Abstract:Nonviral transfection is one of the modem methods for the incorporation of foreign genes into cells. This process involves uptake of foreign genetic material by the cell and further trafficking through the cytoplasm to the nucleus. Elucidation of cytoplasmic pathways of transfection complexes can be useful to improve already existing gene delivery systems or to establish new systems. To monitor transfection complexes in the cell during transfection, we elaborated a method for the visualization of transfection complexes by introducing digoxigenin-labelled nucleotides into foreign DNA followed by detection of digoxigenin label with the use of antibodies directed against digoxigenin. This procedure allowed the visualization of DNA in transfection complexes and to monitor these complexes in cells during transfection.
Keywords:Digoxigenin, DNA-Labeling, H1-mediated Transfection, Immunocytochemistry
Source:Acta Histochemica
Page Range:49-55
Date:1 February 2000
Official Publication:https://doi.org/10.1078/0065-1281-00539
PubMed:View item in PubMed

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