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More than 1,000 putative new human signalling proteins revealed by EST data mining

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Creators Name:Schultz, J. and Doerks, T. and Ponting, C.P. and Copley, R.R. and Bork, P.
Journal Title:Nature Genetics
Journal Abbreviation:Nat Genet
Volume:25
Number:2
Page Range:201-204
Date:1 June 2000
Keywords:Amino Acid Sequence, Amino Acid Sequence Homology, Automation, Catalytic Domain, Computational Biology, Expressed Sequence Tags, Factual Databases, Human Genome, Internet, Molecular Cloning, Molecular Sequence Data, Monomeric GTP-Binding Proteins, Proteins, Sequence Alignment, Signal Transduction, Software, Tertiary Protein Structure
Abstract:Cloning procedures aided by homology searches of EST databases have accelerated the pace of discovery of new genes, but EST database searching remains an involved and onerous task. More than 1.6 million human EST sequences have been deposited in public databases, making it difficult to identify ESTs that represent new genes. Compounding the problems of scale are difficulties in detection associated with a high sequencing error rate and low sequence similarity between distant homologues. We have developed a new method, coupling BLAST-based searches with a domain identification protocol, that filters candidate homologues. Application of this method in a large- scale analysis of 100 signalling domain families has led to the identification of ESTs representing more than 1,000 novel human signalling genes. The 4,206 publicly available ESTs representing these genes are a valuable resource for rapid cloning of novel human signalling proteins. For example, we were able to identify ESTs of at least 106 new small GTPases, of which 6 are likely to belong to new subfamilies. In some cases, further analyses of genomic DNA led to the discovery of previously unidentified full- length protein sequences. This is exemplified by the in silico cloning (prediction of a gene product sequence using only genomic and EST sequence data) of a new type of GTPase with two catalytic domains.
ISSN:1061-4036
Publisher:Nature Publishing Group (U.S.A.)
Item Type:Article

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