Helmholtz Gemeinschaft


Interleukin-7/B7.1-encoding adenoviruses induce rejection of transplanted but not nontransplanted tumors

Item Type:Article
Title:Interleukin-7/B7.1-encoding adenoviruses induce rejection of transplanted but not nontransplanted tumors
Creators Name:Willimsky, G. and Blankenstein, T.
Abstract:Most cancer vaccine trials are based on efficacy studies against transplanted mouse tumors that poorly reflect the clinical situation. We constructed adenoviruses expressing interleukin-7 and B7.1 and tested their therapeutic efficacy after transfer into established transplanted and nontransplanted 3-methylcholanthrene-induced tumors. The adenoviruses efficiently induced rejection of transplanted tumors, leaving behind systemic immunity. Against nontransplanted tumors of similar size, there were almost no therapeutic effects. This result was not due to the site of tumor development, tumor type, general immune suppression, or differences in transduction efficacy. Adenoviral expression of β-galactosidase as a surrogate antigen in nontransplanted tumors induced cytotoxic T cells that were unable to quantitatively reach the tumor site. Based on rigorous mouse models and an effective in situ immunization procedure, it is suggested that cancer vaccines can be effective, if at all, against 'minimal residual disease'; additional experimental procedures must be found against established nontransplanted tumors.
Keywords:Adenoviridae, Beta-Galactosidase, CD80 Antigens, Cultured Tumor Cells, Cytotoxic T-Lymphocytes, Experimental Neoplasms, Gene Transfer Techniques, Graft Rejection, Heterologous Transplantation, Inbred BALB C Mice, Interleukin-7, Neoplasm Transplantation, Animals, Mice
Source:Cancer Research
Publisher:American Association for Cancer Research
Page Range:685-692
Date:1 February 2000
Official Publication:http://cancerres.aacrjournals.org/cgi/content/abstract/60/3/685
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library