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Expression of nitric oxide synthase in kidney macula densa cells

Item Type:Article
Title:Expression of nitric oxide synthase in kidney macula densa cells
Creators Name:Mundel, P. and Bachmann, S. and Bader, M. and Fischer, A. and Kummer, W. and Mayer, B. and Kriz, W.
Abstract:The distribution of nitric oxide synthase (NOS), the enzyme by which NO is generated from L-arginine, was investigated in rat kidney. The indirect immunofluorescence technique using a polyclonal antibody against type I NOS was applied, followed by the histochemical NADPH diaphorase staining technique on the same sections in order to demonstrate the enzymatic activity of NOS. Macula densa cells were strongly stained by both techniques, demonstrating abundant NOS in the cytoplasm of these cells. In addition, these findings were confirmed by nonradioactive in situ hybridization, thus demonstrating the corresponding messenger RNA in macula densa cells as well. Our findings provide the morphological basis for a possible role of NO as a mediator substance in signal transfer from distal tubular fluid to glomerular arterioles.
Keywords:Amino Acid Oxidoreductases, Juxtaglomerular Apparatus, Nitric Oxide Synthase, Messenger RNA, Animals, Rats
Source:Kidney International
ISSN:0085-2538
Publisher:Nature Publishing Group (U.S.A.)
Volume:42
Number:4
Page Range:1017-1019
Date:1 October 1992
Official Publication:https://doi.org/10.1038/ki.1992.382
PubMed:View item in PubMed

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