An electrochemical method for quantification of the radical scavenging activity of SOD

Item Type:	Article
Title:	An electrochemical method for quantification of the radical scavenging activity of SOD
Creators Name:	Lisdat, F. and Ge, B. and Reszka, R. and Kozniewska, E.
Abstract:	An electrochemical technique for the determination of enzymatic superoxide scavenging activity is described. In amperometric mode of sensor operation the oxidation current was altered by additions of superoxide dismutase (SOD). The 50% inhibition value (I50) corresponded to 0.165 U of the standard photometric test of McCord and Fridovich. Influence by inert proteins was much less than in the standard method: The SOD/BSA ratio was 4 x 10(5). Potentially interfering substances, e.g. ascorbic acid and glutathione, were tested. Plasma samples of rats which were treated with modified SOD during cerebral ischaemia, were assayed with the new method and the standard photometric test. A good correlation was found for the analysis in these physiological samples (r = 0.97).
Source:	Fresenius Journal of Analytical Chemistry
ISSN:	0937-0633
Volume:	365
Number:	6
Page Range:	494-498
Date:	1999
Official Publication:	https://doi.org/10.1007/s002160051511

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