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DNA methyltransferase is actively retained in the cytoplasm during early development

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Item Type:Article
Title:DNA methyltransferase is actively retained in the cytoplasm during early development
Creators Name:Cardoso, M.C. and Leonhardt, H.
Abstract:The overall DNA methylation level sharply decreases from the zygote to the blastocyst stage despite the presence of high levels of DNA methyltransferase (Dnmt1). Surprisingly, the enzyme is localized in the cytoplasm of early embryos despite the presence of several functional nuclear localization signals. We mapped a region in the NH(2)-terminal, regulatory domain of Dnmt1 that is necessary and sufficient for cytoplasmic retention during early development. Altogether, our results suggest that Dnmt1 is actively retained in the cytoplasm, which prevents binding to its DNA substrate in the nucleus and thereby contributes to the erasure of gamete-specific epigenetic information during early mammalian development.
Keywords:Cytoplasmic Retention, DNA Methylation, DNA Methyltransferase, Genomic Imprinting, Preimplantation Development , Animals, Mice
Source:Journal of Cell Biology
ISSN:0021-9525
Publisher:Rockefeller University Press (U.S.A.)
Volume:147
Number:1
Page Range:25-32
Date:4 October 1999
Additional Information:Copyright (c) 1999 by The Rockefeller University Press
Official Publication:http://www.jcb.org/cgi/content/abstract/147/1/25
PubMed:View item in PubMed

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