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DNA methyltransferase is actively retained in the cytoplasm during early development

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Official URL:http://www.jcb.org/cgi/content/abstract/147/1/25
PubMed:View item in PubMed
Creators Name:Cardoso, M.C. and Leonhardt, H.
Journal Title:Journal of Cell Biology
Journal Abbreviation:J Cell Biol
Volume:147
Number:1
Page Range:25-32
Date:4 October 1999
Keywords:Cytoplasmic Retention, DNA Methylation, DNA Methyltransferase, Genomic Imprinting, Preimplantation Development , Animals, Mice
Abstract:The overall DNA methylation level sharply decreases from the zygote to the blastocyst stage despite the presence of high levels of DNA methyltransferase (Dnmt1). Surprisingly, the enzyme is localized in the cytoplasm of early embryos despite the presence of several functional nuclear localization signals. We mapped a region in the NH(2)-terminal, regulatory domain of Dnmt1 that is necessary and sufficient for cytoplasmic retention during early development. Altogether, our results suggest that Dnmt1 is actively retained in the cytoplasm, which prevents binding to its DNA substrate in the nucleus and thereby contributes to the erasure of gamete-specific epigenetic information during early mammalian development.
ISSN:0021-9525
Publisher:Rockefeller University Press (U.S.A.)
Additional Information:Copyright (c) 1999 by The Rockefeller University Press
Item Type:Article

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