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Neurotractin, a novel neurite outgrowth-promoting Ig-like protein that interacts with CEPU-1 and LAMP

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Item Type:Article
Title:Neurotractin, a novel neurite outgrowth-promoting Ig-like protein that interacts with CEPU-1 and LAMP
Creators Name:Marg, A. and Sirim, P. and Spaltmann, F. and Plagge, A. and Kauselmann, G. and Buck, F. and Rathjen, F.G. and Bruemmendorf, T.
Abstract:The formation of axon tracts in nervous system histogenesis is the result of selective axon fasciculation and specific growth cone guidance in embryonic development. One group of proteins implicated in neurite outgrowth, fasciculation, and guidance is the neural members of the Ig superfamily (IgSF). In an attempt to identify and characterize new proteins of this superfamily in the developing nervous system, we used a PCR-based strategy with degenerated primers that represent conserved sequences around the characteristic cysteine residues of Ig-like domains. Using this approach, we identified a novel neural IgSF member, termed neurotractin. This GPI-linked cell surface glycoprotein is composed of three Ig-like domains and belongs to the IgLON subgroup of neural IgSF members. It is expressed in two isoforms with apparent molecular masses of 50 and 37 kD, termed L-form and S-form, respectively. Monoclonal antibodies were used to analyze its biochemical features and histological distribution. Neurotractin is restricted to subsets of developing commissural and longitudinal axon tracts in the chick central nervous system. Recombinant neurotractin promotes neurite outgrowth of telencephalic neurons and interacts with the IgSF members CEPU-1 (KD = 3 x 10(-8) M) and LAMP. Our data suggest that neurotractin participates in the regulation of neurite outgrowth in the developing brain.
Keywords:Cell Adhesion, Development, Ig Superfamily, Neurite Outgrowth, Nervous System, Animals, Cricetinae, Chickens
Source:Journal of Cell Biology
Publisher:Rockefeller University Press
Page Range:865-876
Date:17 May 1999
Additional Information:Copyright (c) 1999 by The Rockefeller University Press
Official Publication:http://www.jcb.org/cgi/content/abstract/145/4/865
PubMed:View item in PubMed

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