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Mammalian mitochondrial ribosomal proteins. N-terminal amino acid sequencing, characterization, and identification of corresponding gene sequencing

Item Type:Article
Title:Mammalian mitochondrial ribosomal proteins. N-terminal amino acid sequencing, characterization, and identification of corresponding gene sequencing
Creators Name:Goldschmidt-Reisin, S. and Kitakawa, M. and Herfurth, E. and Wittmann-Liebold, B. and Grohmann, L. and Graack, H.J.
Abstract:The integrity of healthy mitochondria is supposed to depend largely on proper mitochondrial protein biosynthesis. Mitochondrial ribosomal proteins (MRPs) are directly involved in this process. To identify mammalian mitochondrial ribosomal proteins and their corresponding genes, we purified mature rat MRPs and determined 12 different N-terminal amino acid sequences. Using this peptide information, data banks were screened for corresponding DNA sequences to identify the genes or to establish consensus cDNAs and to characterize the deduced MRP open reading frames. Eight different groups of corresponding mammalian MRPs constituted from human, mouse, and rat origin were identified. Five of them show significant sequence similarities to bacterial and/or yeast mitochondrial ribosomal proteins. However, MRPs are much less conserved in respect to the amino acid sequence among species than cytoplasmic ribosomal proteins of eukaryotes and bacteria.
Keywords:Amino Acid Sequence, Complementary DNA, Consensus Sequence, Conserved Sequence, Exons, Expressed Sequence Tags, Introns, Mitochondria, Molecular Sequence Data, Peptide Fragments, Ribosomal Proteins, Sequence Alignment, Sequence Analysis, Species Specificity, Animals, Mice, Rats
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:273
Number:52
Page Range:34828-34836
Date:25 December 1998
Official Publication:https://doi.org/10.1074/jbc.273.52.34828
PubMed:View item in PubMed

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