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Identification of apoptosis-associated proteins in a human Burkitt lymphoma cell line - cleavage of heterogeneous nuclear ribonucleoprotein A1 by caspase 3

Item Type:Article
Title:Identification of apoptosis-associated proteins in a human Burkitt lymphoma cell line - cleavage of heterogeneous nuclear ribonucleoprotein A1 by caspase 3
Creators Name:Brockstedt, E. and Rickers, A. and Kostka, S. and Laubersheimer, A. and Doerken, B. and Wittmann-Liebold, B. and Bommert, K. and Otto, A.
Abstract:Apoptosis or programmed cell death is essential in the process of controlling lymphocyte growth and selection. We identified proteins that are involved in anti-IgM antibody-mediated apoptosis using a subclone of the human Burkitt lymphoma cell line BL60. Apoptosis-associated proteins were detected by high resolution two-dimensional gel electrophoresis on a micropreparative scale. Comparison of the high resolution two-dimensional gel electrophoresis protein patterns from apoptotic and non-apoptotic cells showed differences in approximately 80 spots including protein modifications. Analysis of the predominantly altered proteins was performed by internal Edman microsequencing and/or by peptide mass fingerprinting using matrix-assisted laser desorption/ionization mass spectrometry. Analysis was significantly improved by using new micropreparative high resolution two-dimensional gels employing high protein concentrations. The following 12 apoptosis-associated proteins were identified: heterogeneous nuclear ribonucleoprotein (hnRNP) A1, hnRNP C1/C2, FUSE-binding protein, dUTPase, lymphocyte-specific protein LSP1, UV excision repair protein RAD23 homologue B (HHR23B), 60 S acidic ribosomal protein P0 (L10E), heterochromatin protein 1 homologue alpha (HP1alpha), nucleolin, lamin, neutral calponin, and actin. Fragmentation of actin, hnRNP A1, hnRNP C1/C2, 60 S acidic ribosomal protein P0, lamin, and nucleolin could be inhibited by benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethyl ketone, a selective irreversible inhibitor of CPP32 (caspase 3).
Keywords:Apoptosis, B-Cell Antigen Receptors, Burkitt Lymphoma, Calcium-Binding Proteins, Caspase 3, Caspase Inhibitors, Caspases, Cultured Tumor Cells, DNA Repair Enzymes, DNA-Binding Proteins, Heterogeneous Nuclear RNA, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoprotein Group C, Heterogeneous-Nuclear Ribonucleoproteins, Immunoglobulin M, Immunologic Capping, Immunomagnetic Separation, Lamins, Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry, Microfilament Proteins, Neoplasm Proteins, Non-Histone Chromosomal Proteins, Nuclear Proteins, Oligopeptides, Phosphoproteins, Pyrophosphatases, Ribonucleoproteins, Ribosomal Proteins, RNA-Binding Proteins, Two-Dimensional Gel Electrophoresis
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:28057-28064
Date:23 October 1998
Official Publication:https://doi.org/10.1074/jbc.273.43.28057
PubMed:View item in PubMed

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