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Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site

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Item Type:Article
Title:Efficient transcription of an immunoglobulin kappa promoter requires specific sequence elements overlapping with and downstream of the transcriptional start site
Creators Name:Pelletier, M.R. and Hatada, E.N. and Scholz, G. and Scheidereit, C.
Abstract:The expression of immunoglobulin (Ig) genes depends on tissue-specific elements in the promoter and enhancer regions of light chain and heavy chain genes. In contrast to the complex modular character of Ig enhancers, the promoters appear to be simple, depending primarily on a conserved TATA box and octamer elements. We have analyzed the role of proximal sequences for Igkappa promoter function. Igkappa promoter transcription critically depends on initiator-like sequences and on a downstream element located at +24 to +39 relative to the start site. Replacement of these sequences resulted in strong reduction of promoter activity. In vitro, these elements were found to be more effective in extracts of lymphoid than of non-lymphoid origin. Deletion of the downstream and initiation site regions had a comparable effect on promoter activity to obliteration of the TATA box or octamer element. The downstream sequence was bound by two nuclear proteins, identical to the previously identified Ig-specific C5 and C6 complexes. Whereas C5 is found in HeLa cells and in lymphoid cells, C6 is lymphoid specific. Thus, further specific sequences in addition to the previously characterized elements, the octamer and the TATA box, are required for efficient kappa promoter expression in B lymphocytes.
Keywords:Base Sequence, Consensus Sequence, COS Cells, Gene Deletion, Genetic Enhancer Elements, Genetic Promoter Regions, Genetic Transcription, HeLa Cells, Immunoglobulin Genes, Immunoglobulin kappa-Chains, Molecular Sequence Data, Mutagenesis, Nuclear Proteins, TATA Box, TFII Transcription Factors, Transcription Factor TFIID, Transfection, Animals, Mice
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press (U.K.)
Volume:25
Number:20
Page Range:3995-4003
Date:15 October 1997
Official Publication:http://nar.oxfordjournals.org/cgi/content/abstract/25/20/3995
PubMed:View item in PubMed

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