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A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer

Item Type:Article
Title:A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer
Creators Name:Sellrie, F. and Schenk, J.A. and Behrsing, O. and Boettger, V. and Micheel, B.
Abstract:Mimotope peptides - peptides which mimic the binding of a hapten to its corresponding monoclonal antibody - were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope.
Keywords:Competitive Immunoassay, Enzyme Conjugate, Fluorescein, Mimotope, Animals, Mice
Source:Journal of Immunological Methods
ISSN:0022-1759
Publisher:Elsevier (The Netherlands)
Volume:261
Number:1-2
Page Range:141-144
Date:1 January 2002
Official Publication:https://doi.org/10.1016/S0022-1759(01)00561-0
PubMed:View item in PubMed

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