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Human hematopoiesis in murine embryos after injecting human cord blood-derived hematopoietic stem cells into murine blastocysts

Item Type:Article
Title:Human hematopoiesis in murine embryos after injecting human cord blood-derived hematopoietic stem cells into murine blastocysts
Creators Name:Harder, F. and Henschler, R. and Junghahn, I. and Lamers, M.C. and Mueller, A.M.
Abstract:At different developmental stages, candidate human hematopoletic stem cells (HSCs) are present within the CD34+ CD38- population. By means of xenotransplantation, such CD34+CD38- cells were recently shown to engraft the hematopoietic system of fetal sheep and nonobese diabetic severe combined immunodeficient adult mice. Here it is demonstrated that, after their injection into murine blastocysts, human cord blood (CB)-derived CD34+ and CD34+ CD38- cells repopulate the hematopoietic tissues of nonimmunocompromised murine embryos and that human donor contribution can persist to adulthood. It is further observed that human hematopoietic progenitor cells are present in murine hematopoietic tissues of midgestational chimeric embryos and that progeny of the injected human HSCs activate erythroid-specific gene expression. Thus, the early murine embryo provides a suitable environment for the survival and differentiation of human CB CD34+ CD38- cells.
Keywords:Blastocyst, CD34 Antigens, Cell Differentiation, Cell Lineage, Cell Survival, Chimera, Developmental Gene Expression Regulation, Embryo Transfer, Fetal Blood, Gestational Age, Globins, Hematopoiesis, Hematopoietic System, Heterologous Transplantation, Inbred C57BL Mice, Microinjections, Animals, Mice
Source:Blood
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Volume:99
Number:2
Page Range:719-721
Date:15 January 2002
Official Publication:https://doi.org/10.1182/blood.V99.2.719
PubMed:View item in PubMed

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