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Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization

Item Type:Article
Title:Specific metaphase and interphase detection of the breakpoint region in 8q24 of Burkitt lymphoma cells by triple-color fluorescence in situ hybridization
Creators Name:Ried, T. and Lengauer, C. and Cremer, T. and Wiegant, J. and Raap, A.K. and van der Ploeg, M. and Groitl, P. and Lipp, M.
Abstract:Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma cell line J1 was used for the specific delineation of this breakpoint in individual tumor cells. With this approach, tumor-specific breakpoints in translocation chromosomes can be detected at all stages of the cell cycle with high specificity.
Keywords:Burkitt Lymphoma, Chromosome Banding, Human, Pair 2 Chromosomes, Human, Pair 8 Chromosomes, DNA Probes, Fluorescent Dyes, Interphase, Fluorescence Microscopy, Nucleic Acid Hybridization, Oncogenes, Plasmids, Genetic Translocation, Cultured Tumor Cells
Source:Genes Chromosomes & Cancer
ISSN:1045-2257
Publisher:Wiley (U.S.A.)
Volume:4
Number:1
Page Range:69-74
Date:1 January 1992
Official Publication:https://doi.org/10.1002/gcc.2870040109
PubMed:View item in PubMed

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