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Transcriptional regulation of the HIV-1 promoter by NF-kappa B in vitro

Item Type:Article
Title:Transcriptional regulation of the HIV-1 promoter by NF-kappa B in vitro
Creators Name:Kretzschmar, M. and Meisterernst, M. and Scheidereit, C. and Li, G. and Roeder, R.G.
Abstract:NF-kappa B, purified from HeLa cell cytosol, and a recombinant p50 subunit of NF-kappa B alone (expressed in and purified from bacteria) both stimulated transcription from the HIV-1 promoter in vitro (at least up to 15-fold). A deletion analysis of the p50 subunit revealed that transcriptional activation was mediated by the conserved c-rel-related domain. I kappa B-beta (or a related protein), which binds to the p65 but not the p50 subunit of NF-kappa B, inhibited stimulation by natural NF-kappa B but not by recombinant p50. Experiments employing a purified transcription system revealed that efficient induction of transcription by both natural NF-kappa B or recombinant p50 required a cofactor fraction in addition to the general initiation factors. Combined with DNA-binding experiments, these studies suggest a role of p50 homodimers in transcriptional activation of certain promoters, with a possible preference for those carrying symmetric NF-kappa B recognition sites, and a potential role of I kappa B-beta in direct transcriptional regulation within the nucleus.
Keywords:NF-kappa B, I kappa B, Cofactor USA, HIV-1 Promoter, In Vitro Transcription
Source:Genes & Development
ISSN:0890-9369
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Volume:6
Number:5
Page Range:761-774
Date:May 1992
Official Publication:https://doi.org/10.1101/gad.6.5.761
PubMed:View item in PubMed

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