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Functional interaction of beta-catenin with the transcription factor LEF-1

Item Type:Article
Title:Functional interaction of beta-catenin with the transcription factor LEF-1
Creators Name:Behrens, J. and von Kries, J.P. and Kuehl, M. and Bruhn, L. and Wedlich, D. and Grosschedl, R. and Birchmeier, W.
Abstract:The cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton, and they participate in the wnt/wingless signal pathway. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend. Microinjection of LEF-1 into XenoPus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus.
Keywords:beta Catenin, Cadherins, Cell Line, Cell Nucleus, Cytoskeletal Proteins, DNA, DNA-Binding Proteins, Escherichia Coli, Lymphoid Enhancer-Binding Factor 1, Messenger RNA, Molecular Cloning, Nucleic Acid Conformation, Protein Binding, Recombinant Proteins, Saccharomyces Cerevisiae, Trans-Activators, Transcription Factors, Xenopus Proteins, Animals, Drosophila, Xenopus
Publisher:Nature Publishing Group
Page Range:638-642
Date:15 August 1996
Official Publication:https://doi.org/10.1038/382638a0
PubMed:View item in PubMed

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