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Cell cycle regulation of nuclear factor p92 DNA binding activity by novel phase specific inhibitors

Item Type:Article
Title:Cell cycle regulation of nuclear factor p92 DNA binding activity by novel phase specific inhibitors
Creators Name:Grinstein, E. and Weinert, I. and Droese, B. and Pagano, M. and Royer, H.D.
Abstract:The nuclear factor p92, originally discovered by its interaction with the human papillomavirus type 18 enhancer, is a cellular protein whose activity is restricted to S phase in human primary fibroblasts. The human papillomavirus type 18 p92 binding sequence confers enhancer activity on a heterologous promoter, suggesting that p92 acts as a transcription factor. We have identified a class of nuclear inhibitory proteins, I-92s, which noncovalently associate with p92 but not with other transcription factors such as AP1, E2F, or NF-kappaB. Different I-92s occur in G1, G2, and G0, while no I-92 is detectable in S phase. Phase-specific inhibitors, therefore, are responsible for the cell cycle dependence of p92 activity and provide a novel mechanism linking transcription factor regulation with the cell cycle.
Keywords:Base Sequence, Binding Sites, Cell Cycle, Cell Nucleus, Cultured Cells, DNA-Binding Proteins, Fibroblasts, Flow Cytometry, Genetic Enhancer Elements, Genetic Promoter Regions, HeLa Cells, Molecular Sequence Data, Nuclear Proteins, Oligodeoxyribonucleotides, Papillomaviridae, Recombinant Proteins, S Phase, Simian Virus 40, Transcription Factors, Transfection
Source:Journal of Biological Chemistry
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:9215-9222
Date:19 April 1996
Official Publication:https://doi.org/10.1074/jbc.271.16.9215
PubMed:View item in PubMed

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