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| Item Type: | Article |
|---|---|
| Title: | The C-terminus of the prototypical M2 muscarinic receptor localizes to the mitochondria and regulates cell respiration under stress conditions |
| Creators Name: | Fasciani, I. and Petragnano, F. and Wang, Z. and Edwards, R. and Telugu, N. and Pietrantoni, I. and Zabel, U. and Zauber, H. and Grieben, M. and Terzenidou, M.E. and Di Gregorio, J. and Pellegrini, C. and Santini, S. and Taddei, A.R. and Pohl, B. and Aringhieri, S. and Carli, M. and Aloisi, G. and Marampon, F. and Charlesworth, E. and Roman, A. and Diecke, S. and Flati, V. and Giorgi, F. and Amicarelli, F. and Tobin, A.B. and Scarselli, M. and Tokatlidis, K. and Rossi, M. and Lohse, M.J. and Annibale, P. and Maggio, R. |
| Abstract: | Muscarinic acetylcholine receptors are prototypical G protein-coupled receptors (GPCRs), members of a large family of 7 transmembrane receptors mediating a wide variety of extracellular signals. We show here, in cultured cells and in a murine model, that the carboxyl terminal fragment of the muscarinic M(2) receptor, comprising the transmembrane regions 6 and 7 (M(2)tail), is expressed by virtue of an internal ribosome entry site localized in the third intracellular loop. Single-cell imaging and import in isolated yeast mitochondria reveals that M2tail, whose expression is up-regulated in cells undergoing integrated stress response, does not follow the normal route to the plasma membrane, but is almost exclusively sorted to the mitochondria inner membrane: here, it controls oxygen consumption, cell proliferation, and the formation of reactive oxygen species (ROS) by reducing oxidative phosphorylation. Crispr/Cas9 editing of the key methionine where cap-independent translation begins in human-induced pluripotent stem cells (hiPSCs), reveals the physiological role of this process in influencing cell proliferation and oxygen consumption at the endogenous level. The expression of the C-terminal domain of a GPCR, capable of regulating mitochondrial function, constitutes a hitherto unknown mechanism notably unrelated to its canonical signaling function as a GPCR at the plasma membrane. This work thus highlights a potential novel mechanism that cells may use for controlling their metabolism under variable environmental conditions, notably as a negative regulator of cell respiration. |
| Keywords: | Cell Proliferation, Cell Respiration, HEK293 Cells, Induced Pluripotent Stem Cells, Mitochondria, Muscarinic M2 Receptor, Oxidative Phosphorylation, Oxygen Consumption, Physiological Stress, Reactive Oxygen Species, Animals, Mice |
| Source: | PLoS Biology |
| ISSN: | 1544-9173 |
| Publisher: | Public Library of Science |
| Volume: | 22 |
| Number: | 4 |
| Page Range: | e3002582 |
| Date: | 29 April 2024 |
| Official Publication: | https://doi.org/10.1371/journal.pbio.3002582 |
| PubMed: | View item in PubMed |
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