Intranasal delivery of low-dose anti-CD124 antibody enhances treatment of chronic rhinosinusitis with nasal polyps

Item Type:	Article
Title:	Intranasal delivery of low-dose anti-CD124 antibody enhances treatment of chronic rhinosinusitis with nasal polyps
Creators Name:	Wu, J. and Jones, N. and Chao, P.H. and Chan, V. and Hohenwarter, L. and Wu, A. and Bergamo, M. and Rodríguez-Rodríguez, C. and Saatchi, K. and Liang, A. and Häfeli, U.O. and Tan, Z. and Hedtrich, S. and Andrew, L.J. and Li, S.D.
Abstract:	Frequent injections of anti-CD124 monoclonal antibody (αCD124) over long periods of time are used to treat chronic rhinosinusitis with nasal polyps (CRSwNP). Needle-free, intranasal administration (i.n.) of αCD124 is expected to provide advantages of localized delivery, improved efficacy, and enhanced medication adherence. However, delivery barriers such as the mucus and epithelium in the nasal tissue impede penetration of αCD124. Herein, two novel protamine nanoconstructs: allyl glycidyl ether conjugated protamine (Nano-P) and polyamidoamine-linked protamine (Dendri-P) were synthesized and showed enhanced αCD124 penetration through multiple epithelial layers compared to protamine in mice. αCD124 was mixed with Nano-P or Dendri-P and then intranasally delivered for the treatment of severe CRSwNP in mice. Micro-CT and pathological changes in nasal turbinates showed that these two nano-formulations achieved ∼50 % and ∼40 % reductions in nasal polypoid lesions and eosinophil count, respectively. Both nano-formulations provided enhanced efficacy in suppressing nasal and systemic Immunoglobulin E (IgE) and nasal type 2 inflammatory biomarkers, such as interleukin 13 (IL-13) and IL-25. These effects were superior to those in the protamine formulation group and subcutaneous (s.c.) αCD124 given at a 12.5-fold higher dose. Intranasal delivery of protamine, Nano-P, or Dendri-P did not induce any measurable toxicities in mice.
Keywords:	Intranasal Administration, Monoclonal Antibodies, Chronic Disease, Inbred BALB C Mice, Nasal Polyps, Protamines, Rhinitis, Rhinosinusitis, Sinusitis / Drug Therapy, Animals, Mice
Source:	Biomaterials
ISSN:	0142-9612
Publisher:	Elsevier
Volume:	308
Page Range:	122567
Date:	July 2024
Official Publication:	https://doi.org/10.1016/j.biomaterials.2024.122567
PubMed:	View item in PubMed

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