![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
|
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
7MB | |
![[img]](http://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supplemental Information)
54MB |
| Item Type: | Article |
|---|---|
| Title: | Basal delamination during mouse gastrulation primes pluripotent cells for differentiation |
| Creators Name: | Sato, N. and Rosa, V.S. and Makhlouf, A. and Kretzmer, H. and Sampath Kumar, A. and Grosswendt, S. and Mattei, A.L. and Courbot, O. and Wolf, S. and Boulanger, J. and Langevin, F. and Wiacek, M. and Karpinski, D. and Elosegui-Artola, A. and Meissner, A. and Zernicka-Goetz, M. and Shahbazi, M.N. |
| Abstract: | The blueprint of the mammalian body plan is laid out during gastrulation, when a trilaminar embryo is formed. This process entails a burst of proliferation, the ingression of embryonic epiblast cells at the primitive streak, and their priming toward primitive streak fates. How these different events are coordinated remains unknown. Here, we developed and characterized a 3D culture of self-renewing mouse embryonic cells that captures the main transcriptional and architectural features of the early gastrulating mouse epiblast. Using this system in combination with microfabrication and in vivo experiments, we found that proliferation-induced crowding triggers delamination of cells that express high levels of the apical polarity protein aPKC. Upon delamination, cells become more sensitive to Wnt signaling and upregulate the expression of primitive streak markers such as Brachyury. This mechanistic coupling between ingression and differentiation ensures that the right cell types become specified at the right place during embryonic development. |
| Keywords: | Pluripotency, Embryonic Stem Cells, Gastrulation, Differentiation, Mouse Embryo, 3D Culture, Delamination, Morphogenesis, Wnt Signaling, Proliferation, Animals, Mice |
| Source: | Developmental Cell |
| ISSN: | 1534-5807 |
| Publisher: | Elsevier / Cell Press |
| Date: | 4 April 2024 |
| Official Publication: | https://doi.org/10.1016/j.devcel.2024.03.008 |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
Download Statistics
Download Statistics
