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Ion mobility-tandem mass spectrometry of mucin-type O-glycans

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Item Type:Article
Title:Ion mobility-tandem mass spectrometry of mucin-type O-glycans
Creators Name:Bechtella, L. and Chunsheng, J. and Fentker, K. and Ertürk, G.R. and Safferthal, M. and Polewski, Ł. and Götze, M. and Graeber, S.Y. and Vos, G.M. and Struwe, W.B. and Mall, M.A. and Mertins, P. and Karlsson, N.G. and Pagel, K.
Abstract:The dense O-glycosylation of mucins plays an important role in the defensive properties of the mucus hydrogel. Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn's disease. The inherent complexity of glycans and the diversity in the O-core structure constitute fundamental challenges for the analysis of mucin-type O-glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying O-glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map O-glycosylation features associated with disease.
Keywords:Analytical Biochemistry, Glycosylation, Mass Spectrometry
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:2611
Date:23 March 2024
Official Publication:https://doi.org/10.1038/s41467-024-46825-4
PubMed:View item in PubMed

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