Helmholtz Gemeinschaft


Characterization of anthracycline-induced cardiotoxicity by diffusion tensor magnetic resonance imaging

PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
PDF (Supplementary Information) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:Characterization of anthracycline-induced cardiotoxicity by diffusion tensor magnetic resonance imaging
Creators Name:Lohr, D. and Thiele, A. and Stahnke, M. and Braun, V.M. and Klopfleisch, R. and Klein, O. and Dresen, S. and Landmesser, U. and Foryst-Ludwig, A. and Kintscher, U. and Schreiber, L.M. and Beyhoff, N.
Abstract:Anthracyclines are highly potent anti-cancer drugs, but their clinical use is limited by severe cardiotoxic side effects. The impact of anthracycline-induced cardiotoxicity (AIC) on left ventricular (LV) microarchitecture and diffusion properties remains unknown. This study sought to characterize AIC by cardiovascular magnetic resonance diffusion tensor imaging (DTI). Mice were treated with Doxorubicin (DOX; n = 16) for induction of AIC or saline as corresponding control (n = 15). Cardiac function was assessed via echocardiography at the end of the study period. Whole hearts (n = 8 per group) were scanned ex vivo by high-resolution DTI at 7 T. Results were correlated with histopathology and mass spectrometry imaging. Mice with AIC demonstrated systolic dysfunction (LVEF 52 ± 3% vs. 43 ± 6%, P < 0.001), impaired global longitudinal strain (-19.6 ± 2.0% vs. -16.6 ± 3.0%, P < 0.01), and cardiac atrophy (LV mass index [mg/mm], 4.3 ± 0.1 vs. 3.6 ± 0.2, P < 0.01). Regional sheetlet angles were significantly lower in AIC, whereas helix angle and relative helicity remained unchanged. In AIC, fractional anisotropy was increased (0.12 ± 0.01 vs. 0.14 ± 0.02, P < 0.05). DOX-treated mice displayed higher planar and less spherical anisotropy (C(Planar) 0.07 ± 0.01 vs. 0.09 ± 0.01, P < 0.01; C(Spherical) 0.89 ± 0.01 vs. 0.87 ± 0.02, P < 0.05). C(Planar) and C(Spherical) yielded good discriminatory power to distinguish between mice with and without AIC (c-index 0.91 and 0.84, respectively, P for both < 0.05). AIC is associated with regional changes in sheetlet angle but no major abnormalities of global LV microarchitecture. The geometric shape of the diffusion tensor is altered in AIC. DTI may provide a new tool for myocardial characterization in patients with AIC, which warrants future clinical studies to evaluate its diagnostic utility.
Keywords:Anthracyclines, Cancer Therapy-Related Cardiac Dysfunction, Cardiac Atrophy, Cardiotoxicity, Chemotherapy, Diffusion Tensor Magnetic Resonance Imaging, Animals, Mice
Source:Basic Research in Cardiology
Publisher:Springer Nature
Date:14 March 2024
Official Publication:https://doi.org/10.1007/s00395-024-01039-z
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library