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| Item Type: | Article |
|---|---|
| Title: | MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution |
| Creators Name: | Mertens, T.F., Liebheit, A.T., Ehl, J., Köhler, R., Rakhymzhan, A., Woehler, A., Katthän, L., Ebel, G., Liublin, W., Kasapi, A., Triantafyllopoulou, A., Schulz, T.J., Niesner, R.A. and Hauser, A.E. |
| Abstract: | Analyzing immune cell interactions in the bone marrow is vital for understanding hematopoiesis and bone homeostasis. Three-dimensional analysis of the complete, intact bone marrow within the cortex of whole long bones remains a challenge, especially at subcellular resolution. We present a method that stabilizes the marrow and provides subcellular resolution of fluorescent signals throughout the murine femur, enabling identification and spatial characterization of hematopoietic and stromal cell subsets. By combining a pre-processing algorithm for stripe artifact removal with a machine-learning approach, we demonstrate reliable cell segmentation down to the deepest bone marrow regions. This reveals age-related changes in the marrow. It highlights the interaction between CX(3)CR1(+) cells and the vascular system in homeostasis, in contrast to other myeloid cell types, and reveals their spatial characteristics after injury. The broad applicability of this method will contribute to a better understanding of bone marrow biology. |
| Keywords: | Imaging, Imaging the Immune System, Osteoimmunology, Animals, Mice |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 15 |
| Number: | 1 |
| Page Range: | 1764 |
| Date: | 26 February 2024 |
| Official Publication: | https://doi.org/10.1038/s41467-024-45827-6 |
| PubMed: | View item in PubMed |
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