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TF-FVIIa PAR2-β-arrestin in mouse signaling sustains organ dysfunction in coxsackievirus B3 infection

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Item Type:Article
Title:TF-FVIIa PAR2-β-arrestin in mouse signaling sustains organ dysfunction in coxsackievirus B3 infection
Creators Name:Kespohl, M. and Goetzke, C.C. and Althof, N. and Bredow, C. and Kelm, N. and Pinkert, S. and Bukur, T. and Bukur, V. and Grunz, K. and Kaur, D. and Heuser, A. and Mülleder, M. and Sauter, M. and Klingel, K. and Weiler, H. and Berndt, N. and Gaida, M.M. and Ruf, W. and Beling, A.
Abstract:BACKGROUND: Accumulating evidence implicates the activation of G-protein-coupled PARs (protease-activated receptors) by coagulation proteases in the regulation of innate immune responses. METHODS: Using mouse models with genetic alterations of the PAR2 signaling platform, we have explored contributions of PAR2 signaling to infection with coxsackievirus B3, a single-stranded RNA virus provoking multiorgan tissue damage, including the heart. RESULTS: We show that PAR2 activation sustains correlates of severe morbidity-hemodynamic compromise, aggravated hypothermia, and hypoglycemia-despite intact control of the virus. Following acute viral liver injury, canonical PAR2 signaling impairs the restoration process associated with exaggerated type I IFN (interferon) signatures in response to viral RNA recognition. Metabolic profiling in combination with proteomics of liver tissue shows PAR2-dependent reprogramming of liver metabolism, increased lipid droplet storage, and gluconeogenesis. PAR2-sustained hypodynamic compromise, reprograming of liver metabolism, as well as imbalanced IFN responses are prevented in β-arrestin coupling-deficient PAR2 C-terminal phosphorylation mutant mice. Thus, wiring between upstream proteases and immune-metabolic responses results from biased PAR2 signaling mediated by intracellular recruitment of β-arrestin. Importantly, blockade of the TF (tissue factor)-FVIIa (coagulation factor VIIa) complex capable of PAR2 proteolysis with the NAPc2 (nematode anticoagulant protein c2) mitigated virus-triggered pathology, recapitulating effects seen in protease cleavage-resistant PAR2 mice. CONCLUSIONS: These data provide insights into a TF-FVIIa signaling axis through PAR2-β-arrestin coupling that is a regulator of inflammation-triggered tissue repair and hemodynamic compromise in coxsackievirus B3 infection and can potentially be targeted with selective coagulation inhibitors.
Keywords:Heart Failure, Infections, Inflammation, Myocarditis, Proteomics, Animals, Mice
Source:Arteriosclerosis Thrombosis and Vascular Biology
Publisher:American Heart Association
Page Range:843-865
Date:April 2024
Official Publication:https://doi.org/10.1161/ATVBAHA.123.320157
PubMed:View item in PubMed

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