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| Item Type: | Article |
|---|---|
| Title: | Anti-aquaporin-4 immune complex stimulates complement-dependent Th17 cytokine release in neuromyelitis optica spectrum disorders |
| Creators Name: | Nishiyama, S. and Seok, J.M. and Wright, A.E. and Lotan, I. and Mikami, T. and Drosu, N.C. and Bobrowski-Khoury, N. and Anderson, M.R. and Bilodeau, P.A. and Schindler, P. and Paul, F. and Aoki, M. and Yeaman, M.R. and Levy, M. |
| Abstract: | Proinflammatory cytokines, such as (IL: interleukin) IL-6 and IL-17A, and complement fixation are critical in the immunopathogenesis of neuromyelitis optica spectrum disorders (NMOSD). Blocking the IL-6 receptor or the C5 complement pathway reduces relapse risk. However, the role of interleukin (IL)-6 and complement in aquaporin-4 (AQP4) autoimmunity remains unclear. To investigate the role of the anti-AQP4 immunoglobulin (AQP4-IgG)/AQP4 immunocomplex on the induction and profile of ex vivo cytokine and surface marker expression in peripheral blood mononuclear cells (PBMC) culture. Isolated PBMCs obtained from 18 patients with AQP4-IgG-seropositive-NMOSD (8 treatment-naive, 10 rituximab-treated) or ten healthy controls were cultured with AQP4-immunocomplex with or without complement. Changes in PBMC surface markers and cytokine expression were profiled using flow cytometry and ELISA. PBMCs derived from treatment-naive NMOSD patients stimulated with a complex mixture of serum complement proteins produced significant elevations of IL-17A and IL-6. Rituximab-treated patients also exhibited higher IL-6 but not IL-17A release. IL-6 and IL-17A elevations are not observed without complement. Co-stimulation of PBMCs with AQP4-IgG/AQP4 immunocomplex and complement prompts a Th17-biased response consistent with the inflammatory paradigm observed in NMOSD. A possible inflammation model is proposed via antigen-specific autoreactive peripheral blood cells, including NK/NKT cells. |
| Keywords: | Antigen-Antibody Complex, Aquaporin 4, Autoantibodies, Complement System Proteins, Cytokines, Immunoglobulin G, Interleukin-17, Interleukin-6, Mononuclear Leukocytes, Neuromyelitis Optica, Rituximab, Rituximab / Therapeutic Use |
| Source: | Scientific Reports |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Volume: | 14 |
| Number: | 1 |
| Page Range: | 3146 |
| Date: | 7 February 2024 |
| Official Publication: | https://doi.org/10.1038/s41598-024-53661-5 |
| PubMed: | View item in PubMed |
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