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| Item Type: | Article |
|---|---|
| Title: | Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| Creators Name: | Maierhof, S.K. and Schinke, C. and Cernoch, J. and Hew, L. and Bruske, L.P. and Fernandez Vallone, V. and Fischer, K. and Stachelscheid, H. and Huehnchen, P. and Endres, M. and Diecke, S. and Telugu, N.S. and Boehmerle, W. |
| Abstract: | NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease. |
| Keywords: | Calcium, CRISPR-Cas Systems, Gene Editing, Gene Knockout Techniques, Induced Pluripotent Stem Cells |
| Source: | Stem Cell Research |
| ISSN: | 1873-5061 |
| Publisher: | Elsevier |
| Volume: | 73 |
| Page Range: | 103253 |
| Date: | December 2023 |
| Official Publication: | https://doi.org/10.1016/j.scr.2023.10325 |
| PubMed: | View item in PubMed |
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