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| Item Type: | Article |
|---|---|
| Title: | Using PrISMa to reveal the interactome of the human claudins family |
| Creators Name: | Perez-Hernandez, D. and Suarez-Artiles, L. and Jones, M.S.O. and Dittmar, G. |
| Abstract: | Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. |
| Keywords: | High-Throughput Screening, Protein Biochemistry, Proteomics, Mass Spectrometry |
| Source: | STAR Protocols |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 4 |
| Number: | 4 |
| Page Range: | 102549 |
| Date: | 15 December 2023 |
| Official Publication: | https://doi.org/10.1016/j.xpro.2023.102549 |
| PubMed: | View item in PubMed |
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