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| Item Type: | Preprint |
|---|---|
| Title: | The synapsin-dependent vesicle cluster is crucial for presynaptic plasticity at a glutamatergic synapse in male mice |
| Creators Name: | Bruentgens, F. and Moreno Velasquez, L. and Stumpf, A. and Parthier, D. and Breustedt, J.M. and Benfenati, F. and Milovanovic, D. and Schmitz, D. and Orlando, M. |
| Abstract: | Synapsins are highly abundant presynaptic proteins that play a crucial role in neurotransmission and plasticity via the clustering of synaptic vesicles. The synapsin III isoform is usually downregulated after development, but in hippocampal mossy fiber boutons it persists in adulthood. Mossy fiber boutons express presynaptic forms of short- and long-term plasticity, which are thought to underlie different forms of learning. Previous research on synapsins at this synapse focused on synapsin isoforms I and II. Thus, a complete picture regarding the role of synapsins in mossy fiber plasticity is still missing. Here, we investigated presynaptic plasticity at hippocampal mossy fiber boutons by combining electrophysiological field recordings and transmission electron microscopy in a mouse model lacking all synapsin isoforms. We found decreased short-term plasticity - i.e. decreased facilitation and post-tetanic potentiation - but increased long-term potentiation in male synapsin triple knockout mice. At the ultrastructural level, we observed more dispersed vesicles and a higher density of active zones in mossy fiber boutons from knockout animals. Our results indicate that all synapsin isoforms, including synapsin III, are required for fine regulation of short- and long-term presynaptic plasticity at the mossy fiber synapse. |
| Keywords: | Animals, Mice |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2023.08.08.549335 |
| Date: | 8 August 2023 |
| Official Publication: | https://doi.org/10.1101/2023.08.08.549335 |
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