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A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels

Item Type:Article
Title:A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels
Creators Name:Swain, M. and Ross, N.W.
Abstract:A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.
Keywords:Silver Staining, Protein, Sodium Dodecyl Sulfate-Polyacryl-Amide Gel Electrophoresis
Source:Electrophoresis
ISSN:0173-0835
Publisher:Wiley (Germany)
Volume:16
Number:6
Page Range:948-951
Date:1 June 1995
Official Publication:https://doi.org/10.1002/elps.11501601159
PubMed:View item in PubMed

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