Polyglycerol coatings of glass vials for protein resistance

Item Type:	Article
Title:	Polyglycerol coatings of glass vials for protein resistance
Creators Name:	Höger, K. and Becherer, T. and Qiang, W. and Haag, R. and Friess, W. and Küchler, S.
Abstract:	Proteins are surface active molecules which undergo non-specific adsorption when getting in contact with surfaces such as the primary packaging material. This process is critical as it may cause a loss of protein content or protein aggregation. To prevent unspecific adsorption, protein repellent coatings are of high interest. We describe the coating of industrial relevant borosilicate glass vials with linear methoxylated polyglycerol, hyperbranched polyglycerol, and hyperbranched methoxylated polyglycerol. All coatings provide excellent protein repellent effects. The hyperbranched, non-methoxylated coating performed best. The protein repellent properties were maintained also after applying industrial relevant sterilization methods (⩾200 °C). Marginal differences in antibody stability between formulations stored in bare glass vials and coated vials were detected after 3 months storage; the protein repellent effect remained largely stable. Here, we describe a new material suitable for the coating of primary packaging material of proteins which significantly reduces the protein adsorption and thus could present an interesting new possibility for biomedical applications.
Keywords:	Protein Adsorption, Hyperbranched Polyglycerol, Surface Coating, Glass Vials, PEG, Packaging Material
Source:	European Journal of Pharmaceutics and Biopharmaceutics
ISSN:	0939-6411
Publisher:	Elsevier
Volume:	85
Number:	3 Pt A
Page Range:	756-764
Date:	November 2013
Official Publication:	https://doi.org/10.1016/j.ejpb.2013.04.005
PubMed:	View item in PubMed

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