Effect of delivery platforms structure on the epidermal antigen transport for topical vaccination

Item Type:	Article
Title:	Effect of delivery platforms structure on the epidermal antigen transport for topical vaccination
Creators Name:	Sonzogni, A.S. and Yealland, G. and Kar, M. and Wedepohl, S. and Gugliotta, L.M. and Gonzalez, V.D.G. and Hedtrich, S. and Calderón, M. and Minari, R.J.
Abstract:	Transdermal immunization is highly attractive because of the skin's accessibility and unique immunological characteristics. However, it remains a relatively unexplored route of administration because of the great difficulty of transporting antigens past the outermost layer of skin, the stratum corneum. In this article, the abilities of three poly( N-vinylcaprolactam) (PVCL)-based thermoresponsive assemblies-PVCL hydrogels and nanogels plus novel film forming PVCL/acrylic nanogels-to act as protein delivery systems were investigated. Similar thermal responses were observed in all systems, with transition temperatures close to 32 °C, close to that of the skin surface. The investigated dermal delivery systems showed no evidence of cytotoxicity in human fibroblasts and were able to load and release ovalbumin (OVA), a well-studied antigen, in a temperature-dependent manner in vitro. The penetration of OVA into ex vivo human skin following topical application was evaluated, where enhanced skin delivery was seen for the OVA-loaded PVCL systems relative to administration of the protein alone. The distinct protein release and skin penetration profiles observed for the different PVCL assemblies were here discussed on the basis of their structural differences.
Keywords:	Antigens, Caprolactam, Cutaneous Administration, Dermis, Drug Carriers, Epidermis, Hydrogels, Nanoparticles, Ovalbumin, Polyethylene Glycols, Polyethyleneimine, Polymers, Skin, Skin Absorption, Temperature, Vaccination
Source:	Biomacromolecules
ISSN:	1526-4602
Publisher:	American Chemical Society
Volume:	19
Number:	12
Page Range:	4607-4616
Date:	10 December 2018
Official Publication:	https://doi.org/10.1021/acs.biomac.8b01307
PubMed:	View item in PubMed

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