Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis

Item Type:	Article
Title:	Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis
Creators Name:	Jevtić, M. and Löwa, A. and Nováčková, A. and Kováčik, A. and Kaessmeyer, S. and Erdmann, G. and Vávrová, K. and Hedtrich, S.
Abstract:	Dermal fibroblasts seem critical for epidermal maturation and differentiation and recent work demonstrated that diseased fibroblasts may drive pathophysiological processes. Nevertheless, still very little is known about the actual crosstalk between epidermal keratinocytes and dermal fibroblasts and the impact of dermal fibroblasts on epidermal maturation and differentiation. Aiming for a more fundamental understanding of the impact of the cellular crosstalk between keratinocytes and fibroblasts on the skin homeostasis, we generated full-thickness skin equivalents with and without fibroblasts and subsequently analysed them for the expression of skin differentiation markers, their barrier function, skin lipid content and epidermal cell signalling. Skin equivalents without fibroblasts consistently showed an impaired differentiation and dysregulated expression of skin barrier and tight junction proteins, increased skin permeability, and a decreased skin lipid/protein ratio. Most interestingly, impaired Ras/Raf/ERK/MEK signalling was evident in skin equivalents without fibroblasts. Our data clearly indicate that the epidermal-dermal crosstalk between keratinocytes and fibroblasts is critical for adequate skin differentiation and that fibroblasts orchestrate epidermal differentiation processes.
Keywords:	Keratinocyte-Fibroblast Crosstalk, Skin Equivalents, Epidermal Differentiation, Skin Homeostasis
Source:	Biochimica et Biophysica Acta - Molecular Cell Research
ISSN:	0167-4889
Publisher:	Elsevier
Volume:	1867
Number:	8
Page Range:	118722
Date:	August 2020
Official Publication:	https://doi.org/10.1016/j.bbamcr.2020.118722
PubMed:	View item in PubMed

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