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CRISPR/Cas9 screen in gastric cancer patient-derived organoids reveals KDM1A-NDRG1 axis as a targetable vulnerability

Item Type:Article
Title:CRISPR/Cas9 screen in gastric cancer patient-derived organoids reveals KDM1A-NDRG1 axis as a targetable vulnerability
Creators Name:Mircetic, J. and Camgöz, A. and Abohawya, M. and Ding, L. and Dietzel, J. and Tobar, S.G. and Paszkowski-Rogacz, M. and Seidlitz, T. and Schmäche, T. and Mehnert, M.C. and Sidorova, O. and Weitz, J. and Buchholz, F. and Stange, D.E.
Abstract:Viability CRISPR screens have proven indispensable in parsing genome function. However, their application in new, more physiologically relevant culturing systems like patient-derived organoids (PDOs) has been much slower. To probe epigenetic contribution to gastric cancer (GC), the third leading cause of cancer-related deaths worldwide, the first negative selection CRISPR screen in GC PDOs that faithfully preserve primary tumor characteristics is performed. Extensive quality control measurements showing feasibility of CRISPR screens in primary organoid culture are provided. The screen reveals the histone lysine demethylase-1A (KDM1A) to constitute a GC vulnerability. Both genetic and pharmacological inhibition of KDM1A cause organoid growth retardation. Further, it is shown that most of KDM1A cancer-supporting functions center on repression of N-myc downstream regulates gene-1 (NDRG1). De-repression of NDRG1 by KDM1A inhibitors (KDM1Ai) causes inhibition of Wnt signaling and a strong G1 cell cycle arrest. Finally, by profiling 20 GC PDOs, it is shown that NDRG1 upregulation predicts KDM1Ai response with 100% sensitivity and 82% specificity in the tested cohort. Thus, this work pioneers the use of negative selection CRISPR screens in patient-derived organoids, identifies a marker of KDM1Ai response, and accordingly a cohort of patients who may benefit from such therapy.
Keywords:CRISPR Screen, Epigenetic Regulators, Gastric Cancer, KDM1A-NDRG1 Axis, Patient-Derived Organoids
Source:Small Methods
Page Range:e2201605
Date:20 June 2023
Official Publication:https://doi.org/10.1002/smtd.202201605
PubMed:View item in PubMed

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