![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
|
PDF (Original Article)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
9MB | |
![[img]](http://edoc.mdc-berlin.de/style/images/fileicons/other.png) |
Other (Supporting Information)
202MB |
| Item Type: | Article |
|---|---|
| Title: | The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation |
| Creators Name: | Meier, M. and Gupta, M. and Akgül, S. and McDougall, M. and Imhof, T. and Nikodemus, D. and Reuten, R. and Moya-Torres, A. and To, V. and Ferens, F. and Heide, F. and Padilla-Meier, G.P. and Kukura, P. and Huang, W. and Gerisch, B. and Mörgelin, M. and Poole, K. and Antebi, A. and Koch, M. and Stetefeld, J. |
| Abstract: | Netrin-1 is a bifunctional chemotropic guidance cue that plays key roles in diverse cellular processes including axon pathfinding, cell migration, adhesion, differentiation, and survival. Here, we present a molecular understanding of netrin-1 mediated interactions with glycosaminoglycan chains of diverse heparan sulfate proteoglycans (HSPGs) and short heparin oligosaccharides. Whereas interactions with HSPGs act as platform to co-localise netrin-1 close to the cell surface, heparin oligosaccharides have a significant impact on the highly dynamic behaviour of netrin-1. Remarkably, the monomer-dimer equilibrium of netrin-1 in solution is abolished in the presence of heparin oligosaccharides and replaced with highly hierarchical and distinct super assemblies leading to unique, yet unknown netrin-1 filament formation. In our integrated approach we provide a molecular mechanism for the filament assembly which opens fresh paths towards a molecular understanding of netrin-1 functions. |
| Keywords: | Biophysical Chemistry, Caenorhabditis Elegans, Intracellular Signalling Peptides and Proteins, X-Ray Crystallography |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 14 |
| Number: | 1 |
| Page Range: | 1226 |
| Date: | 3 March 2023 |
| Official Publication: | https://doi.org/10.1038/s41467-023-36692-w |
| PubMed: | View item in PubMed |
Repository Staff Only: item control page
Download Statistics
Download Statistics
