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Massively parallel identification of mRNA localization elements in primary cortical neurons

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Item Type:Article
Title:Massively parallel identification of mRNA localization elements in primary cortical neurons
Creators Name:Mendonsa, S. and von Kügelgen, N. and Dantsuji, S. and Ron, M. and Breimann, L. and Baranovskii, A. and Lödige, I. and Kirchner, M. and Fischer, M. and Zerna, N. and Bujanic, L. and Mertins, P. and Ulitsky, I. and Chekulaeva, M.
Abstract:Cells adopt highly polarized shapes and form distinct subcellular compartments in many cases due to the localization of many mRNAs to specific areas, where they are translated into proteins with local functions. This mRNA localization is mediated by specific cis-regulatory elements in mRNAs, commonly called ‘zipcodes’. Although there are hundreds of localized mRNAs, only a few zipcodes have been characterized. Here we describe a novel neuronal zipcode identification protocol (N-zip) that can identify zipcodes across hundreds of 3′ untranslated regions. This approach combines a method of separating the principal subcellular compartments of neurons—cell bodies and neurites—with a massively parallel reporter assay. N-zip identifies the let-7 binding site and (AU)n motif as de novo zipcodes in mouse primary cortical neurons. Our analysis also provides, to our knowledge, the first demonstration of an miRNA affecting mRNA localization and suggests a strategy for detecting many more zipcodes.
Keywords:Animals, Mice
Source:Nature Neuroscience
ISSN:1097-6256
Publisher:Nature Publishing Group
Date:16 January 2023
Official Publication:https://doi.org/10.1038/s41593-022-01243-x
PubMed:View item in PubMed

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