Rapid nuclear deadenylation of mammalian messenger RNA
| Item Type: | Article |
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| Title: | Rapid nuclear deadenylation of mammalian messenger RNA |
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| Creators Name: | Alles, J. and Legnini, I. and Pacelli, M. and Rajewsky, N. |
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| Abstract: | Poly(A) tails protect RNAs from degradation and their deadenylation rates determine RNA stability. Although poly(A) tails are generated in the nucleus, deadenylation of tails has mostly been investigated within the cytoplasm. Here, we combined long-read sequencing with metabolic labeling, splicing inhibition and cell fractionation experiments to quantify, separately, the genesis and trimming of nuclear and cytoplasmic tails in vitro and in vivo. We present evidence for genome-wide, nuclear synthesis of tails longer than 200 nt, which are rapidly shortened after transcription. Our data suggests that rapid deadenylation is a nuclear process, and that different classes of transcripts and even transcript isoforms have distinct nuclear tail lengths. For example, many long-noncoding RNAs retain long poly(A) tails. Modelling deadenylation dynamics predicts nuclear deadenylation about 10 times faster than cytoplasmic deadenylation. In summary, our data suggests that nuclear deadenylation might be a key mechanism for regulating mRNA stability, abundance, and subcellular localization. |
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| Keywords: | Animals, Mice |
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| Source: | iScience |
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| ISSN: | 2589-0042 |
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| Publisher: | Cell Press |
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| Volume: | 26 |
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| Number: | 1 |
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| Page Range: | 105878 |
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| Date: | 20 January 2023 |
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| Official Publication: | https://doi.org/10.1016/j.isci.2022.105878 |
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| PubMed: | View item in PubMed |
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| Related to: | |
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